In vitro antioxidant and antiradical properties of Hippophae rhamnoides L.

Hippophae rhamnoides L. is a member of the Elaeagnaceae family, different parts of it, especially juice and oil of the fruits, have been used for the treatment of several diseases in traditional medicine in various countries. In the present study we investigated the possible antiradical and antioxidant activities of the hexanoic extract obtained from ripe fruit of H. rhamnoides L. (HRE) in in vitro conditions using different antioxidant tests: ferric thiocyanate method, reducing power, metal chelating activities and 1,1-diphenyl-2-picryl-hydrazyl (DPPH·) free radical scavenging. Results of experiment revealed that HRE has a concentration-dependent antioxidant effect. Total antioxidant activity was measured according to ferric thiocyanate method. At the concentration of 10, 20 and 50 μg/mL, the inhibitory effect of HRE on peroxidation of linoleic acid emulsion was found to be 36.8, 50.8 and 68.7 %, respectively. On the other hand, percentage inhibition of α-tocopherol was found to be 8.9 %. In addition, HRE was also effective in reducing power, metal chelating activities and DPPH· free radical scavenging experiments. These various antioxidant activities were compared to α-tocopherol, EDTA or trolox which references antioxidants. The results obtained from present study clearly showed thad HRE had marked in vitro antioxidant and antiradical activites.