High-performance Liquid Chromatographic Determination of Paracetamol, Propyphenazone, and Caffeine in Pharmaceutical Formulations

This study proposes a liquid chromatography (RPHPLC) method for quantitative analysis of the most widely prescribed combination of Paracetamol, Propyphenazone and Caffeine in tablet dosage form in presence of Rasagiline as an internal standard. The chromatography was performed on a Gracesmart C18 column (5μm, 250mm×4.6mm i.d.) using the mixture of water and 2-propanol in the ratio 80:20 v/v as mobile phase. The pH of aqueous phase was adjusted to 3.0 with 1% o-phosphoric acid and a flow rate was maintained at 1.5ml/min. The analytes were detected by measuring the absorbance at 210nm. The chromatographic analysis time for each sample was kept about 20min as Rasagiline, Paracetamol, Caffeine and Propyphenazone were eluted at 1.63, 2.36, 3.17 and 11.22 min respectively. The calibration plots were obtained between 3–90μg/ml for Paracetamol, 1.5–45μg/ml for Propyphenazone and 0.5–15μg/ml for Caffeine. The method was validated in accordance with ICH norms and method was found to be specific, accurate, precise and easy to use for routine analysis.