Development and Validation of Inductively Coupled Plasma Atomic Emission Spectroscopy [ICP-AES] Analytical Method for Estimation of Cisplatin in Biological Samples

Wagle, Shruti Akshay; Jain, Darshana; Rathod, Sudha; Athawale, Rajani; Bajaj, Amrita

doi:10.5530/ijper.51.4s.113

Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 51 | Issue 4s | 2017 | pp. s783–s789Open access

Development and Validation of Inductively Coupled Plasma Atomic Emission Spectroscopy [ICP-AES] Analytical Method for Estimation of Cisplatin in Biological Samples

¹^*,
²,
¹,
³,
⁴

¹ Oriental College of Pharmacy, Sector 2, Plot No 3, 4, 5, Sanpada (W), Navi Mumbai, Maharashtra, INDIA.
² C.U.Shah College of Pharmacy, S.N.D.T Women’s University, Santacruz (W), Mumbai, Maharashtra, INDIA.
³ Principal K.M Kundnani College of Pharmacy, Plot No. 23, Jote Joy Building, Rambhau Salgaonkar Road, Cuffe Parade, Mumbai, Maharashtra, INDIA.
⁴ SVKM’s Dr. Bhanuben Nanavati College of Pharmacy, V.M Road, Vile Parle (W), Mumbai, Maharashtra, INDIA.

Correspondence: Shruti Akshay Wagle
Oriental College of Pharmacy, Sector 2, Plot No 3, 4, 5, Sanpada (W), Navi Mumbai, Maharashtra, INDIA.
Email: wagleshruti85@gmail.com

Published:: Dec 25, 2017
Received:: Jun 30, 2017
Accepted:: Oct 14, 2017
DOI:: 10.5530/ijper.51.4s.113

How to cite

Wagle, S. A., Jain, D., Rathod, S., Athawale, R., & Bajaj, A. (2017). Development and Validation of Inductively Coupled Plasma Atomic Emission Spectroscopy [ICP-AES] Analytical Method for Estimation of Cisplatin in Biological Samples. Indian Journal of Pharmaceutical Education and Research, 51(4s), s783–s789. https://doi.org/10.5530/ijper.51.4s.113

Abstract

Objective: Cisplatin, a popular anti-neoplastic agent is employed as a first line treatment for variety of cancers. Majority of the analytical methods reported for Cisplatin are either complex or not suitable for routine analysis of the drug. Hence, there is a need for development of suitable analytical technique like ICP-AES for quantitation of Cisplatin in complex matrices and biological samples. Methods: Cisplatin was analyzed in the present study based on its single step conversion to platinum metal with aqua regia. Results: The developed method was validated and was found to be linear in the range of 0.375 to 15 μg/ml with a regression coefficient of 0.992. The percent recoveries of Cisplatin from plasma and tissue samples ranged from 95 to 99%. Discussion: A simple, low cost, sensitive, one step ICP-AES technique was developed and validated for bioanalytical estimation of Cisplatin.

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Article metadata

Title	Development and Validation of Inductively Coupled Plasma Atomic Emission Spectroscopy [ICP-AES] Analytical Method for Estimation of Cisplatin in Biological Samples
Authors	Shruti Akshay Wagle; Darshana Jain; Sudha Rathod; Rajani Athawale; Amrita Bajaj
Affiliations	Oriental College of Pharmacy, Sector 2, Plot No 3, 4, 5, Sanpada (W), Navi Mumbai, Maharashtra, INDIA.; C.U.Shah College of Pharmacy, S.N.D.T Women’s University, Santacruz (W), Mumbai, Maharashtra, INDIA.; Principal K.M Kundnani College of Pharmacy, Plot No. 23, Jote Joy Building, Rambhau Salgaonkar Road, Cuffe Parade, Mumbai, Maharashtra, INDIA.; SVKM’s Dr. Bhanuben Nanavati College of Pharmacy, V.M Road, Vile Parle (W), Mumbai, Maharashtra, INDIA.
Corresponding author	wagleshruti85@gmail.com
Journal	Indian Journal of Pharmaceutical Education and Research
Volume / Issue	Vol. 51, Issue 4s (2017)