Determination of Antioxidant and Anti-inflammatory Potentials of Selected Therapeutic Plants Aqueous Extract and their Formulation for Wound Healing

Background

Since ancient times, medicinal plants with antioxidant and anti-inflammatory abilities have been utilized for relieving a variety of health problems. Individual herbs, however, are inadequate to provide the necessary therapeutic effect. It will have a greater therapeutic impact with less toxicity when tailored as a multiple herb mixture in a specific ratio. In Ayurveda, this combination of herbs is known as polyherbalism.

Aim

The current research aimed to create a polyherbal combination from extracts of Anogeissus latifolia (AL), Centella asiatica (CA), Curcuma longa (CL), Glinus oppositifolius (GO) and Soymida febrifuga (SF) in order to produce such a treatment. The locals in Chhattisgarh state use these chosen medicinal herbs to cure wounds.

Materials and Methods

Spectrophotometric techniques were used in this work to assess the wound-healing capability of the extracts and their polyherbal combination. The total polyphenols were optimized using Folin’s ciocalteu and aluminium chloride techniques. The antioxidant capacity was assessed using the DPPH and FRAP tests. Anti-inflammatory activity was determined using protein denaturation tests, whereas wound healing potential was determined using phenol-protein precipitation assays.

Results

All experiments yielded significant (p<0.05) findings from the extracts and their combinations, however aqueous extracts and their polyherbal mixes were shown to be superior to petroleum ether and methanol extracts and their polyherbal mixtures.

Conclusion

In conclusion, the studied polyherbal mixture is being employed for the different formulation processes as well as for further in vitro and in vivo research.