Anti-Inflammatory Activity Potential of Arglabin in LPS-Stimulated RAW 264.7 Macrophages

Background

Uncontrolled inflammation, however, adds to the pathophysiology of many chronic diseases even though it is a vital defense mechanism in health. Despite being necessary for reducing inflammation, anti-inflammatory medications have a number of negative effects. Thus, the purpose of the current work was to examine the anti-inflammatory properties of Arglabin (AGN), a sesquiterpene lactone that is isolated from Artemisia species and belongs to the guaianolide class. AGN, a renewable substance employed in the synthesis of novel chemicals, is one of the practically accessible sesquiterpene lactones.

Materials and Methods

Using the murine macrophage RAW 264.7 as a model, we examined the anti-inflammatory properties of AGN by preventing the generation of mediators and cytokines that promote inflammation, as well as their ability to regulate Reactive Oxygen Species (ROS). Using Lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, we evaluated AGN anti-inflammatory effects using cytotoxic activity, Nitric Oxide (NO) assays and enzyme-linked immunosorbent assays.

Results and Discussion

AGN suppressed the generation of ROS and NO. It was shown that in RAW 264.7 cells treated with LPS, AGN decreased released pro-inflammatory cytokines, including TNF-α and IL-6. Furthermore, AGN at various concentrations up to 20 μM was not cytotoxic. Additionally, the data show that AGN reduces inflammation by suppressing Prostaglandin E2 (PGE2).

Conclusion

In summary, these findings indicate that AGN may serve as a drug for inflammation to treat severe inflammatory conditions by inhibiting the production of pro-inflammatory cytokines and successfully reducing macrophage activation.