Iron in Highbush Blueberries and It’s Effect in Total Antioxidant Status (TAS) Assay

Radhika, Kota; Ara, DerMarderosian

Research ArticlePharmacognosy ResearchVol. 1 | Issue 4 | 2009 | pp. 166–171Open access

Iron in Highbush Blueberries and It’s Effect in Total Antioxidant Status (TAS) Assay

¹^*,
²

¹ Dept. of Chemistry & Biochemistry, University of the Sciences in Philadelphia, PA, U.S.A, rkota@mail.usp.edu
² Dept. of Chemistry & Biochemistry, University of the Sciences in Philadelphia, PA, U.S.A, a.dermar@usp.edu

Correspondence: Kota Radhika
Dept. of Chemistry & Biochemistry, University of the Sciences in Philadelphia, PA, U.S.A, rkota@mail.usp.edu
Email: rkota@mail.usp.edu

Published:: Jan 1, 2009
Received:: May 19, 2009
Accepted:: Jun 3, 2009

How to cite

Radhika, K., & Ara, D. (2009). Iron in Highbush Blueberries and It’s Effect in Total Antioxidant Status (TAS) Assay. Pharmacognosy Research, 1(4), 166–171.

Abstract

Blueberries are well known for their antioxidant property, due to the polyphenolics present in these fruits. The antioxidant activity of blueberries is usually measured by colorimetric assays involving metals such as iron, copper. Total Antioxidant Status (TAS) assay was previously studied on blueberry fractions which has metmyoglobulin (protein bound iron) in its +3 oxidation state to assess the antioxidant activity of compounds. Studies showed that the amount of iron in highbush blueberries is too low to have a significant effect on the antioxidant activity measured by the TAS assay. A short comparative study was also performed using ascorbic acid in TAS assay and bathocuproine copper complex assay for a quick, inexpensive, rapid analysis of crude screening for antioxidant compounds.

Keywords

Subject

Article metadata

Title	Iron in Highbush Blueberries and It’s Effect in Total Antioxidant Status (TAS) Assay
Authors	Kota Radhika; DerMarderosian Ara
Affiliations	Dept. of Chemistry & Biochemistry, University of the Sciences in Philadelphia, PA, U.S.A, rkota@mail.usp.edu; Dept. of Chemistry & Biochemistry, University of the Sciences in Philadelphia, PA, U.S.A, a.dermar@usp.edu
Corresponding author	rkota@mail.usp.edu
Journal	Pharmacognosy Research
Volume / Issue	Vol. 1, Issue 4 (2009)