HPTLC-Based Phytochemical Profiling and Quality Control Evaluation of Sarivadi Lauha with Relevance to Diabetic Neuropathy

Background

Sarivadi Lauha is a classical Ayurvedic herbo-mineral formulation widely used in the management of metabolic disorders. Standardisation of such formulations is essential to ensure quality, safety, and reproducibility.

Objectives

To evaluate the physicochemical parameters and develop an HPTLC fingerprint profile of Sarivadi Lauha tablets for analytical standardisation.

Materials and Methods

Sarivadi Lauha tablets were evaluated for organoleptic characteristics and physicochemical parameters including pH, loss on drying, ash values, extractive values, hardness, friability, and disintegration time according to Ayurvedic Pharmacopoeia of India guidelines. Methanolic extract of the formulation was analysed by High-Performance Thin Layer Chromatography (HPTLC) using silica gel 60 F254 plates and a mobile phase consisting of toluene: ethyl acetate: formic acid (5:2:0.5 v/v/v). Densitometric scanning was performed at 254 nm and 366 nm.

Results

Physicochemical parameters were within acceptable limits for herbo-mineral formulations. HPTLC analysis revealed multiple well-resolved peaks at characteristic R_f values indicating the presence of phenolic, flavonoid, tannin, coumarin, and terpenoid constituents.

Conclusion

The developed physicochemical standards and HPTLC fingerprint provide reliable analytical parameters for quality control and authentication of Sarivadi Lauha.