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ORIGINAL ARTICLEPharmacognosy ResearchVol. 6 | Issue 3 | 2014 | pp. 234–239Open access
Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites
- 1,2*,
- 1,
- 1,
- 3
- 1 Department of Pharmacology and Pharmaceutical Medicinal Chemistry, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra
- 2 Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan
- 3 Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabaugh, Aurangabad, Maharashtra, India
Published in Pharmacognosy Research
Correspondence: Gaurav Mahesh Doshi
Department of Pharmacology and Pharmaceutical Medicinal Chemistry, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra; Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan
Email: gaurav.pharmacology@gmail.com
Copyright: © 2014 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2014
How to cite
Doshi, G. M., Zine, S. P., Chaskar, P. K., & Une, H. D. (2014). Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites. Pharmacognosy Research, 6(3), 234–239. https://doi.org/10.4103/0974-8490.132601
Abstract
Background
Polyalthia longifolia Thwaites is an important traditional plant in India. Rutin, an active constituent has been reported to possess good amount of pharmacological as well as therapeutic potential.
Objective
The aim of the present study was to find out by analytical techniques how much percentage of rutin is present in the plant leaves’ ethanolic extract by analytical techniques.
Materials and Methods
Shade dried leaves of Polyalthia longifolia were subjected to cold ethanolic extraction followed by monitoring the isolated rutin high-pressure liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC) after carrying out preliminary phytochemical screening.
Results
Extraction yield was found to be 13.94% w/w. Phytochemical screening of the extract showed the presence of flavonoids, steroids, diterpenoids, alkaloids, saponins, tannins and phenolic compounds and mucilage. From the Rf value, the ethanolic extract was found to be having constituent identical to rutin. By HPTLC and HPLC the amount of rutin was found to be 11.60% w/w and 4.03% w/v, respectively.
Conclusion
The active constituent isolated was found to be equal to rutin.
Keywords
Subject
Article metadata
| Title | Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites |
|---|---|
| Authors | Gaurav Mahesh Doshi; Sandeep Prabhakar Zine; Pratip Kashinath Chaskar; Hemant Devidas Une |
| Affiliations | Department of Pharmacology and Pharmaceutical Medicinal Chemistry, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra; Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan; Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabaugh, Aurangabad, Maharashtra, India |
| Corresponding author | gaurav.pharmacology@gmail.com |
| Journal | Pharmacognosy Research |
| Volume / Issue | Vol. 6, Issue 3 (2014) |
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