Simultaneous determination of 11 bioactive compounds in Jaeumganghwa‑tang by high performance liquid chromatography‑diode array detection

Background: Jaeumganghwa‑tang (JEGH) is a traditional Korean herbal medicine for the treatment of chronic bronchitis, nephritis and diabetes mellitus. Objective: A high performance liquid chromatography‑diode array detector (HPLC‑DAD) method was developed for simultaneous determination of 11 major compounds such as 5‑ hydroxymethylfurfural, mangiferin, paeoniflorin, nodakenin, naringin, hesperidin, decursinol, berberine, glycyrrhizin, atractylenolide III and decursin, in JEGH. Materials and Methods: The separation was conducted on Shishedo C18 column with gradient elution of 0.1% trifluoroacetic acid–acetonitrile. Detection of wavelength was set at 205, 250, 280 and 330 nm. Results: The developed analysis showed a good linearity (R2 >0.9997). The range of limit of detection and limit of quantification were observed from 0.04 to 0.43 and from 0.11 to 1.30, respectively. The intra‑ and inter‑day test relative standard deviations (RSD) were less than 3% and the accuracy was 95.98‑108.44%. The recoveries were between 92.75% and 109.19% and RSD range of recoveries was measured from 0.52% to 2.78%. Conclusion: This HPLC‑DAD method can be successfully applied for simultaneous determination of 11 major compounds in JEGH samples.