Open access · Peer reviewed · No APCs
ORIGINAL ARTICLEPharmacognosy MagazineVol. 13 | Issue 49 | 2017 | pp. 175–179Open access
Rapid Analysis of Components in Coptis chinensis Franch by Ultra-Performance Liquid Chromatography with Quadrupole Time-of-Flight Mass Spectrometry
- 1,2,
- 1,2,
- 1,
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- 2,4*,
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- 1 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing, China.
- 2 Laboratory of Cell Pharmacology, College of Pharmaceutical Sciences, Hebei University, Baoding, China.
- 3 Henan University of Traditional Chinese Medicine, Zhengzhou, China.
- 4 Drug Quality Control Key Laboratory of Hebei Province, Hebei University, Baoding, China.
Published in Pharmacognosy Magazine
Correspondence: Yu-xin Liu
Laboratory of Cell Pharmacology, College of Pharmaceutical Sciences, Hebei University, Baoding, China.; Drug Quality Control Key Laboratory of Hebei Province, Hebei University, Baoding, China.
Email: yuxinliu@hbu.edu.cn
Correspondence: Shao-jing Li
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing, China.
Email: shaojingli2004@126.com
Copyright: © 2017 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 6, 2017
- Received:
- Nov 1, 2016
- Accepted:
- Dec 5, 2016
How to cite
Tian, P. P., Zhang, X. X., Wang, H. P., Li, P. L., Liu, Y. X., & Li, S. J. (2017). Rapid Analysis of Components in Coptis chinensis Franch by Ultra-Performance Liquid Chromatography with Quadrupole Time-of-Flight Mass Spectrometry. Pharmacognosy Magazine, 13(49), 175–179. https://doi.org/10.4103/0973-1296.197635
Abstract
Background: Coptis chinensis Franch is a traditional Chinese medical herb. Objective: In this article, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to rapidly, qualitatively, and comprehensively identify the components in Coptis chinensis Franch. Materials and Methods: Chromatographic separation was achieved on an Agilent Zorbax RRHD Eclipse Plus C18 column. The mobile phase consisted of 0.1% formic acid water (A) and 0.1% formic acid acetonitrile (B) with a gradient program. Qualitative analysis was performed on an Agilent 6540 quadrupole time-of-flight mass spectrometer, which was equipped with a Dual AJS ESI source operating in negative mode. Results: A total of 30 alkaloid and non-alkaloid components of Coptis chinensis Franch were identified in only 14 min. Conclusion: This study helped to provide a basis for the quality control of Coptis chinensis Franch.
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Article metadata
| Title | Rapid Analysis of Components in Coptis chinensis Franch by Ultra-Performance Liquid Chromatography with Quadrupole Time-of-Flight Mass Spectrometry |
|---|---|
| Authors | Peng-peng Tian; Xiao-xu Zhang; Hong-ping Wang; Pu-ling Li; Yu-xin Liu; Shao-jing Li |
| Affiliations | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing, China.; Laboratory of Cell Pharmacology, College of Pharmaceutical Sciences, Hebei University, Baoding, China.; Henan University of Traditional Chinese Medicine, Zhengzhou, China.; Drug Quality Control Key Laboratory of Hebei Province, Hebei University, Baoding, China. |
| Corresponding author | yuxinliu@hbu.edu.cn |
| Journal | Pharmacognosy Magazine |
| Volume / Issue | Vol. 13, Issue 49 (2017) |
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