6-Gingerol Prevents Free Transition Metal Ion [Fe (Ii)] Induced Free Radicals Mediated Alterations by In vitro and Ndv Growth in Chicken Eggs by In ovo

Background: The study of free radicals and their reactions is implicated in the pathogenesis of various human diseases. Therefore, antioxidants treatment plays a pivotal role in preventing the free radical‑induced diseases. Objective: The objectives of this study were to examine the protective role of 6‑gingerol from free transition metal ion‑induced alterations by in vitro and Newcastle disease virus (NDV) in chicken eggs by in ovo. Materials and Methods: In this study, lipid peroxidation, DNA sugar oxidation, chelation of ferrous ions, DNA fragmentation, Cytochrome c-oxidation, ferric ion reducing activities and DPPH, superoxide and hydroxyl radicals scavenging activity by ESR studies were examined with 6-gingerol by in vitro. Furthermore, antiviral activity was screened with 6-gingerol by in ovo. Results: In vitro results showed that 254 μM/ml of 6‑gingerol firmly inhibited erythrocyte membrane lipid peroxidation by 85.28% and DNA sugar oxidation by 91.26% when compared with butylated hydroxyanisole 74.42% (554 μM/ml) and 78.30% (824 μM/ml), respectively. Whereas, chelating activity of 6-gingerol was 80.2% which nearly similar to the EDTA activity (50 µg/ml) shown 83.93%. In addition, 6-gingerol (254µM/ml) scavenged the superoxide, hydroxyl, DPPH radicals by 84.96%, 90.45%, and 94.63% respectively and the same was strongly supported with ESR studies. Further, 254 µM/ml of 6-gingerol inhibited the DNA fragmentation, Cytochrome c-oxidation and reducing the ferric ion by in vitro and antiviral activity shown on NDV at 100 µg/ml. Conclusion: This study could enlighten that the 6‑gingerol has a good metal sequester property and it could prevent the free radicals by in vitro and NDV growth in chicken eggs by in ovo.