Optimization of Ultrasonic‑Assisted Extraction of Bioactive Compounds from Sargassum henslowianum using Response Surface Methodology

Objective: This study was to optimize the ultrasound‑assisted extraction of polysaccharides and polyphenols from S. henslowianum and test the biological activity of the extract. Materials and Methods: Response surface methodology was used to optimize extraction conditions, and the antioxidant activity of the extracts was evaluated by radical scavenging assay, α‑Glucosidase inhibition, and cytotoxicity on MCF‑7. Results: The optimal conditions of extracting polysaccharides were shown as following: ultrasonic time for 40 min, ultrasonic power for 330 W, solid‑to‑liquid ratio for 1:36 g/mL, the extraction yield reached 12.63% under above parameters. The optimum conditions of ultrasonic‑assisted extraction of total polyphenols were as following: ultrasonic time for 102 min, ultrasonic power for 377 W, alcohol concentration for 62%, under these conditions, the extraction yield reached 11.45%. Besides, the extracts of polyphenols possessed stronger activity. Conclusion: This study provides the scientific guidance for further exploitation and utilization of S. henslowianum.