In vitro Evaluation of the Effects of Lycopene on Caspase System and Oxidative DNA Damage in High‑Glucose Condition

Background: The present study was planned to investigate the effects of lycopene, on the caspase‑dependent apoptosis in high‑dose glucose (HG)‑treated PC12 cell line. PC12 cells were cultured in vitro. Materials and Methods: HG was prepared as G (250 mM), and lycopene was prepared as L1 (10 μM), L2 (20 μM), and L3 (40 μM). After 6 h of incubation, the cells were exposed to trypsin, and the samples were obtained with freeze/ thaw method. Caspase 3, 8, 9; 8‑hydroxy‑2‑deoxyguanosine (8‑OHdG); and M30 were determined (enzyme‑linked immunosorbent assay). Results: 8‑OHdG increased in L3 (P ≤ 0.001), whereas L1 caused a decrease in HG group (P ≤ 0.001). Caspase‑3 decreased significantly in L1, L2, and L3G compared to control (P ≤ 0.001) group. Caspase‑8 increased significantly in L1, L1G, L2G, and all L3 glucose groups (P ≤ 0.001). There was no difference for Caspase‑9. M30 was not affected by L and HG, which decreased significantly (P ≤ 0.001). Conclusion: As a result, it was determined that, when PC12 cell line was treated with HG, lycopene application had effects on caspase enzymes and DNA damage.