Anti-inflammatory and Antioxidant Activities of Cissus quadrangularis and Cissus repens

• was: NaCl, 118; KCl, 4.7; CaCl2, 2.5; MgSO4, 1.2; KH2PO4, 1.2; NaHCO^1*

1. ¹ ethylenediaminetetraacetic acid, 0.026 and dextrose, 11.1. The solution was maintained at pH 7.4 and gassed with 95% O2 and 5% CO2 at 37°C. The rings were initially stretched to a basal tension of 3.0 g before allowing them to equilibrate in the bathing medium; tissues were allowed to stabilize for 20 min. The contractions were recorded with an isometric vertical force transducer (BIOPAC®) connected to a MP150 Data Analysis Software (Acknowledge®). After the stabilization period, the tissues were stimulated with NE (0.1 μM) during 10 min, and then they were washed with new Krebs solution. This sensitization process of the tissues was repeated three times, with intervals of 30 min, before starting the experiments. The absence of endothelium was confirmed by the lack of the relaxant response induced by the CCh (1 μM) during the last contraction. Finally, for the evaluation period, all tissues were contracted with NE (0.1 μM) and was added to the bath quarter‑log cumulative concentrations (2.16–210 μM) of the mix‑H:N or positive control (CCh [1.13 × 10‑04 to 10 μM]), a cholinergic agonist, for the experiments with endothelium and nifedipine (1.13 × 10‑04 to 10 μM), a calcium channel blocker agent, for experiments without endothelium). Determination of mode of action of mix‑H:N on aortic rings To establish the mode of action of mix‑H:N, after the stabilization and sensitization period, the following experiments were conducted. • In order to know the role of Nitric Oxide (NO) or soluble Guanylyl Cyclase enzyme (sGC) in the vasorelaxant effect of the mix‑H:N.

Published in Pharmacognosy Magazine