A New Stilbene Derivative and Isolated Compounds from Bauhinia pottsii var. pottsii with their Anti‑alpha‑glucosidase Activity

Objectives: Isolation of chemical constituents from B. pottsii var. pottsii and determination of their biological activity on alpha-glucosidase inhibition. Materials and Methods: Chromatographic techniques, enzymatic assay, and molecular docking were done in this study. Results: A new stilbene derivative, 1,3‑benzenediol‑5‑(‑2‑phenylethyl)‑6‑(2’,3’‑butanediol)‑3’, methyl (1) and five known compounds such as 3,4‑dihydroxybenzoic acid (2), quercetin (3), 3‑O‑methylquercetin (4), a mixture of beta‑sitosterol and stigmasterol (5), and 3,4,5‑trimethoxyphenyl‑beta‑D‑glucopyranoside (6) were isolated from B. pottsii var. pottsii. The plant extracts and isolated compounds were determined for anti‑alpha‑glucosidase activity. The results showed that the compounds 2, 3, and 4 were inhibited alpha‑glucosidase enzyme activity with inhibition concentration at 50% (IC50) values of 3.637 mM, 0.486 mM, and 0.292 mM, respectively. While positive control, acarbose exhibited IC50 value as 0.166 mM. The mechanism of action was proved using computer molecular docking. Compound 2 was predicted as mixed‑type inhibitor, whereas compounds 3 and 4 were predicted as competitive inhibitors. Conclusion: This study will be the first report of phytochemical and biological study from B. pottsii var. pottsii including the first report of new compound.