Open access · Peer reviewed · No APCs
Original ArticlePharmacognosy MagazineVol. 16 | Issue 70s | 2020 | pp. S431–S434Open access
Protein Expression Patterns in HEK‑Blue™ ‑ Cells Treated with Clinacanthus nutans Extracts
- 2,
- 3,
- 4,5,
- 4,
- 2,
- 6,
- *,
- 6*
- 1 Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia.
- 2 Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, School of Pharmacy, International Medical University.
- 3 Department of Pharmaceutical Chemistry, School of Pharmacy, International Medical University.
- 4 Centre for Cancer and Stem Cells Research, Institute for Research, Development and Innovation, International Medical University, Kuala Lumpur.
- 5 Health Sciences Division, Abu Dhabi Women’s College, Higher Colleges of Technology, Abu Dhabi, United Arab Emirates.
- 6 Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, School of Pharmacy, International Medical University, Malaysia.
Published in Pharmacognosy Magazine
Correspondence: Ngai‑Paing Tan
Email: ngaipaing@upm.edu.my
Correspondence: Kok‑Song Lai
Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, School of Pharmacy, International Medical University, Malaysia.
Email: lkoksong@hct.ac.ae
Copyright: © 2020 Manuscript Technomedia. This is an open access article.
- Published:
- Aug 28, 2020
- Received:
- Jul 9, 2019
- Accepted:
- Feb 17, 2020
- DOI:
- 10.4103/pm.pm_281_19
How to cite
Yang, S., Leo, T., Mai, C., Chin, S., Lamasudin, D. U., Lim, S., Tan, N., & Lai, K. (2020). Protein Expression Patterns in HEK‑Blue™ ‑ Cells Treated with Clinacanthus nutans Extracts. Pharmacognosy Magazine, 16(70s), S431–S434. https://doi.org/10.4103/pm.pm_281_19
Abstract
Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK‑Blue™‑4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two‑dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK‑Blue™‑4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK‑Blue™‑4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin‑1 was also upregulated in HEK‑Blue™‑4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK‑Blue™‑4 cells and induced the expression of peroxiredoxin‑1, which protects the cells from reactive oxygen species during the inflammation process.
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Article metadata
| Title | Protein Expression Patterns in HEK‑Blue™ ‑ Cells Treated with Clinacanthus nutans Extracts |
|---|---|
| Authors | Shun‑Kai Yang; Teik‑Kee Leo; Chun‑Wai Mai; Swee‑Yee Chin; Dhilia Udie Lamasudin; Swee‑Hua‑Erin Lim; Ngai‑Paing Tan; Kok‑Song Lai |
| Affiliations | Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia.; Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, School of Pharmacy, International Medical University.; Department of Pharmaceutical Chemistry, School of Pharmacy, International Medical University.; Centre for Cancer and Stem Cells Research, Institute for Research, Development and Innovation, International Medical University, Kuala Lumpur.; Health Sciences Division, Abu Dhabi Women’s College, Higher Colleges of Technology, Abu Dhabi, United Arab Emirates.; Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, School of Pharmacy, International Medical University, Malaysia. |
| Corresponding author | ngaipaing@upm.edu.my |
| Journal | Pharmacognosy Magazine |
| Volume / Issue | Vol. 16, Issue 70s (2020) |
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- Naringenin Sensitizes Lung Cancer NCI‑H23 Cells to Radiation by Downregulation of Akt Expression and Metastasis while Promoting Apoptosispp. S229–S235
- Polyphenol‑Enriched Fraction and the Compounds Isolated from Garcinia indica Fruits Ameliorate Obesity through Suppression of Digestive Enzymes and Oxidative Stresspp. S236–S245
- New Phytopharmaceutical Formulations: Development and Characterization of Tablets Containing the Aerial Part of the Plant Pulverized and the Soft Extract from Bidens pilosa Standardized on Rutinpp. S246–S254
- Neurocognitive Investigation of Morinda tinctoria against Amyloid Beta‑Induced Oxidative Insult and Cognitive Impairment in Albino Mice: A Phytotherapeutic Approachpp. S255–S262
- Turcuron: A Standardized Bisacurone‑Rich Turmeric Rhizome Extract for the Prevention and Treatment of Hangover and Alcohol‑Induced Liver Injury in Ratspp. S263–S271
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