Spectrum–Effect Relationships between Fingerprints of Radix Polygoni multiflori‑Achyranthes bidentate and Antiosteoporosis Effect Based on Different Extraction Solvents

Background: Polygonum multiflorum and Achyranthes bidentate are traditional Chinese herbs, which show significant antiosteoporosis (OP) effect. These two herbs are commonly used as a combination in traditional Chinese medicine. Materials and Methods: In this study, the left humerus section of the mice were stained with hematoxylin and eosin staining to establish the spectrum‑effect relationships between the high‑performance liquid chromatography fingerprint of different solvent extracts and the anti‑OP effect. Finally, we analyzed the correlation between the content of main compounds of different solvent extracts and the anti‑OP effect. Results: The compatibility had obvious therapeutic effect on OP induced by retinoic acid. The results of pharmacodynamic analysis showed that the extract had a certain effect on the bone‑specific alkaline phosphatase, the level of tartrate‑resistant acid phosphatase‑5b (TRACP‑5b), and estrogen 2 and had a great influence on TRACP‑5b especially. The results of spectrum‑effect relationships and correlation analysis showed that the level of β‑ecdysterone and tetrahydroxystilbene is negatively correlated with bone resorption index and TRACP‑5b. The higher the content of the two compounds in the combination, the better the effect of treating mouse OP induced by retinoic acid. Conclusion: It is suggested that the compatibility of P. multiflorum and A. bidentata can be used to treat OP, and their mechanism of action is through reducing osteoclastic effect. β‑ecdysterone and tetrahydroxystilbene may be the active components of combination of Polygoni multiflori‑A. bidentate.