Molecular Mechanisms of Dolichandrone serrulata Flower Ethanolic Extract on Antimigration of Human Cervical Cancer Cell Line

Background: Dolichandrone serrulata flower has been known for its antioxidation and anti‑inflammation. Its ability to induce cell death and reduce migration on cancer cell lines has been previously demonstrated. Aim: The present study aimed to investigate the molecular mechanisms involved in the antimigration effect of D. serrulata flower ethanolic extracts of HeLa cell line. Materials and Methods: HeLa cell line viability was assessed by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay. The antimigration of the extract was investigated using a wound healing assay. Its possible molecular mechanism for the migration of HeLa cell line was investigated using a protein array assay. Results: D. serrulata extract caused cell death with CC50 of 232.10 ± 17.16 µg/mL. The result from the wound healing assay demonstrated that D. serrulata extracts at concentrations of 62.5, 125, and 250 µg/mL caused the reduction of the narrowing in the wound site, corresponding to the progress over time. To eradicate the possibility of the cytotoxic effect of the herb, D. serrulata at a concentration of 125 µg/mL was further observed for protein expression using a protein array assay. The result showed the reduction of galactin‑3, vimentin, and fibroblast growth factor‑2 expression compared with the control group. Conclusion: Antimigratory effect of D. serrulata flower ethanolic extracts might be attributable to the reduction of vimentin and fibroblast growth factor‑2 gene expression, but not galactin‑3‑induced apoptosis. Further study is needed to confirm and investigate a more in‑depth pathway on its antimigration effect in the cervical cancer cell line.