Anti‑inflammatory Effect of luteolin‑7‑O‑glucoside via the JAK1/ STAT6/SOCS1 Pathway in Ulcerative Colitis Treatment

Background: Luteolin‑7‑O‑glucoside (Lut‑7‑G) is an effective compound found in plants, such as Patrinia and honeysuckle. It has anti‑inflammatory as well as antioxidant properties; however, its anti‑inflammatory effect on ulcerative colitis (UC) is hardly understoo Objectives: We evaluated the effects of Lut‑7‑G in dextran sodium sulfate (DSS)‑induced UC in mice models, and then explore the underlying mechanism by studying the JAK1/STAT6/SOCS1 signaling pathway. Materials and Methods: Induction of acute colitis in mice was achieved by feeding 2.5% DSS for 7 days. Bodyweight loss, colon length, disease activity index (DAI) score, and spleen index were determined and hematoxylin‑eosin and Periodic Acid‑Schiff staining were performed to study the pathological changes in mouse colons. The inflammatory factor levels were determined by ELISA, JAK1, STAT6, and SOCS1 expression in colon tissues by RT‑qPCR, and signaling pathway proteins by Western blotting. Results: It was found that treatment with Lut‑7‑G reduced the effects of colon shortening and weight loss, DAI score, spleen index, as well as colon inflammation. In addition, it significantly decreased DSS‑induced overexpression of IL‑6, IL‑1β, IL‑18, as well as TNF‑α, and considerably reduced mRNA expression of JAK1 and STAT6 but upregulated the SOCS1 expression. Furthermore, Lut‑7‑G treatment dose‑dependently decreased JAK1 and STAT6 protein expression, and only DSS + Lut‑7‑G (100 mg/kg) could downregulate p‑JAK1 and p‑STAT6 expression and upregulate SOCS1 protein expression. Moreover, Lut‑7‑G (100 mg/kg) was as effective as mesalazine. Conclusion: Lut‑7‑G may regulate the secretion of inflammatory factors and inhibit inflammatory responses through the JAK1/STAT6/SOCS1 pathway, as a determinant in the treatment of UC.