Global yield of the Supercritical CO2 extraction from Cordia verbenacea DC - Anticancer and antimycobacterial activities

In the present work supercritical fluid extraction (SFE) from Cordia verbenacea DC was studied. The effects of pressure and temperature on the global yield were investigated using the Surface Response Methodology. Extracts of C. verbenacea were also prepared by hydrodistillation and Soxhlet using ethanol. The chemical compositions of the extracts were determined by GC-FID. The anticancer and antimycobacterial activities of the extracts were evaluated. The results show that pressure and temperature significantly affected the global yield (pvalue < 0.05). The maximum global yield was obtained at 300 bar and 50°C (4.25 % dry basis – d.b.). The extract obtained by Soxhlet using ethanol showed the highest yield (8.13 ± 0.01 % d.b). β-caryophyllene was identified as the major compound in the volatile fraction. The anticancer activity of the SFE extracts varied with the operational condition. The SFE extract obtained at 200 bar and 40 °C showed the best results and it is similar to that presented by the extracts obtained by hydrodistillation and Soxhlet extraction. The SFE extracts showed a lower antimycobacterial activity.