Open access · Peer reviewed · No APCs
Research ArticlePharmacognosy MagazineVol. 5 | Issue 17 | 2009 | pp. 71–74Open access
A Sensitive Reversed Phase HPLC Method for the Determination of Curcumin
- 1,
- 1*
- 1 Department of Pharmaceutics, Al-Ameen college of Pharmacy, Hosur Road, Bangalore - 560027, India.
Published in Pharmacognosy Magazine
Correspondence: Sarasija Suresh
Department of Pharmaceutics, Al-Ameen college of Pharmacy, Hosur Road, Bangalore - 560027, India.
Email: sarasija_s@hotmail.com
Copyright: © 2009 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2009
- Received:
- Apr 29, 2008
- Accepted:
- Nov 5, 2008
How to cite
Ramshankar, Y. V., & Suresh, S. (2009). A Sensitive Reversed Phase HPLC Method for the Determination of Curcumin. Pharmacognosy Magazine, 5(17), 71–74.
Abstract
A simple, precise, rapid and accurate, binary reverse phase high performance liquid chromatographic method has been developed for the determination of curcumin a natural drugs with short run time. Chromatographic separation was achieved by using Merck C15 (250 cm X 4.6 mm) Column with mobile phase acetonitril: tetrahydrofuran: 2% acetic acid 50:30:20 (2%) was used. The flow rate was 0.7 ml/min. The retention time was 4.587 minutes. The limit of detection and limit of quantification of curcumin were between 3.68 to 8.125 ng/ml for 50-µL injection volumes. The percentage recovery of curcumin was found to be 97.2 to 98.4 and Relative standard deviation was 0.345 % and 1.160 %. The developed HPLC method can therefore be applied to both in vitro studies of curcumin formulations as well as drug estimation in biological samples.
Keywords
Subject
Article metadata
| Title | A Sensitive Reversed Phase HPLC Method for the Determination of Curcumin |
|---|---|
| Authors | Yadav Vivek Ramshankar; Sarasija Suresh |
| Affiliations | Department of Pharmaceutics, Al-Ameen college of Pharmacy, Hosur Road, Bangalore - 560027, India. |
| Corresponding author | sarasija_s@hotmail.com |
| Journal | Pharmacognosy Magazine |
| Volume / Issue | Vol. 5, Issue 17 (2009) |
Also in this issue
- A new antimalarial agent; effect of extracts of Artemisia diffusa against Plasmodium bergheipp. 1–7
- In vitro effects of extract of Senna alata (Ceasalpiniaceae) on the polyamines produced by Leukaemia cells (L1210)pp. 8–13
- Influence of different strains of Agrobacterium rhizogenes on induction of hairy roots and lignan production in Linum tauricum ssp. tauricumpp. 14–18
- Anti-hepatocarcinogenic Ayurvedic herbal remedy reduces the extent of diethylnitrosamine-induced oxidative stress in ratspp. 19–27
- Chemical Composition and Hepato-protective activity of Imperata cylindrica Beauv.pp. 28–36
Readers Also Viewed
Development and Validation of UV/visible Spectrophotometric Method for Estimation of Piroxicam from Bulk and Formulation
Sandip Mohan Honmane, Kunal Rajaram Yadav, Yuvraj Dilip Dange
Apr 23, 2025
Effects of Artificial Intelligence on Academic Performance of Library and Information Science University Students: A Meta-Analysis (2023-2025)
Kayode Sunday John Dada
Aug 6, 2026
Bridging Innovation and Impact: A Multidisciplinary Approach to Contemporary Research Challenges
Mueen Ahmed KK
Aug 11, 2026