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Research ArticlePharmacognosy MagazineVol. 5 | Issue 17 | 2009 | pp. 71–74Open access
A Sensitive Reversed Phase HPLC Method for the Determination of Curcumin
- 1,
- 1*
- 1 Department of Pharmaceutics, Al-Ameen college of Pharmacy, Hosur Road, Bangalore - 560027, India.
Published in Pharmacognosy Magazine
Correspondence: Sarasija Suresh
Department of Pharmaceutics, Al-Ameen college of Pharmacy, Hosur Road, Bangalore - 560027, India.
Email: sarasija_s@hotmail.com
Copyright: © 2009 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2009
- Received:
- Apr 29, 2008
- Accepted:
- Nov 5, 2008
How to cite
Ramshankar, Y. V., & Suresh, S. (2009). A Sensitive Reversed Phase HPLC Method for the Determination of Curcumin. Pharmacognosy Magazine, 5(17), 71–74.
Abstract
A simple, precise, rapid and accurate, binary reverse phase high performance liquid chromatographic method has been developed for the determination of curcumin a natural drugs with short run time. Chromatographic separation was achieved by using Merck C15 (250 cm X 4.6 mm) Column with mobile phase acetonitril: tetrahydrofuran: 2% acetic acid 50:30:20 (2%) was used. The flow rate was 0.7 ml/min. The retention time was 4.587 minutes. The limit of detection and limit of quantification of curcumin were between 3.68 to 8.125 ng/ml for 50-µL injection volumes. The percentage recovery of curcumin was found to be 97.2 to 98.4 and Relative standard deviation was 0.345 % and 1.160 %. The developed HPLC method can therefore be applied to both in vitro studies of curcumin formulations as well as drug estimation in biological samples.
Keywords
Subject
Article metadata
| Title | A Sensitive Reversed Phase HPLC Method for the Determination of Curcumin |
|---|---|
| Authors | Yadav Vivek Ramshankar; Sarasija Suresh |
| Affiliations | Department of Pharmaceutics, Al-Ameen college of Pharmacy, Hosur Road, Bangalore - 560027, India. |
| Corresponding author | sarasija_s@hotmail.com |
| Journal | Pharmacognosy Magazine |
| Volume / Issue | Vol. 5, Issue 17 (2009) |
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