Biological Evaluation of Mercurialis annua Extracts for Possible Antioxidant, Antiproliferative and Cytotoxic Activity

Al-Douri, Nedhal; Al-Jaidi, Bilal Ali; Hammad, Hana Mamoun; Shakya, Ashok Kumar; Alkhalailah, Tahany Jalal Mefleh; Shilbayeh, Sireen Abdul Rahim; Ibrahim, Alnada Abdalla; Venugopala, Katharigatta Narayanaswamy; Kamal, Yoonus Thajudeen

doi:10.5530/ijper.56.3s.156

Original ArticleIndian Journal of Pharmaceutical Education and ResearchVol. 56 | Issue 3s | 2024 | pp. S479–S486Open access

Biological Evaluation of Mercurialis annua Extracts for Possible Antioxidant, Antiproliferative and Cytotoxic Activity

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¹ Department of Pharmacy, Al-Manara College of Medical Sciences, Maysan, Al-Amarah, IRAQ.
² Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Yarmouk University, Irbid, JORDAN.
³ Department of Biological Sciences, School of Sciences, University of Jordan, Amman, JORDAN.
⁴ Faculty of Pharmacy and Medical Sciences, Al Ahliyya Amman University, Amman, JORDAN.
⁵ Department of Pharmaceutical Sciences, Faculty of Pharmacy, Philadelphia University, Amman, JORDAN.
⁶ Department of Pharmacy Practice, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh, SAUDI ARABIA.
⁷ Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology.
⁸ Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, SAUDI ARABIA.
⁹ Department of Pharmacognosy, College of Pharmacy, King Khalid University, Abha, SAUDI ARABIA.

Correspondence: Bilal Ali Al-Jaidi
Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Yarmouk University, Irbid, JORDAN.
Email: bilal.aljaidi@yu.edu.jo

Published:: Jan 1, 2024
Received:: Mar 30, 2022
Accepted:: May 23, 2022
DOI:: 10.5530/ijper.56.3s.156

How to cite

Al-Douri, N., Al-Jaidi, B. A., Hammad, H. M., Shakya, A. K., Alkhalailah, T. J. M., Shilbayeh, S. A. R., Ibrahim, A. A., Venugopala, K. N., & Kamal, Y. T. (2024). Biological Evaluation of Mercurialis annua Extracts for Possible Antioxidant, Antiproliferative and Cytotoxic Activity. Indian Journal of Pharmaceutical Education and Research, 56(3s), S479–S486. https://doi.org/10.5530/ijper.56.3s.156

Abstract

Background: Mercurialis annua has been traditionally used by native people for its diuretic, laxative, antiemetic and antioxidant activity in addition to its usefulness in the treatment of warts, eye problems and microbial infections. Aim: The goal of this work is to study the different extracts of aerial parts of Mercurialis annua collected from the Jordan territories and test them for possible antioxidant, cytotoxic and antiangiogenic activities. Materials and Methods: One kilogram of the dried powdered aerial parts of M. annua, was extracted by maceration with n-hexane, ethyl acetate, and ethanol (96%), respectively. Flavonoids were detected and identified by UV spectrophotometer, TLC, NMR, Mass, and melting point in comparison with the standard. The brine-Shrimp cytotoxicity /Lethality test was performed to measure the LC₅₀ for all M. annua extracts, using Colchicine and chloroform as positive control and negative control, respectively. Both the ethanolic extract and ethyl acetate extract of M. annua were tested for DPPH radical scavenging activity (antioxidant activity). The ethanol extract was evaluated for possible antiproliferative activities against human leukemia (K562), breast cancer (MCF-7), cervical cancer (Hela), lung cancer (A562), and fibroblast cell lines. All the three extracts from M. annua were also evaluated for possible antiangiogenic activity using the ex vivo Rat Aortic assay. Results: The ethanolic extract has shown mild cytotoxic activity, especially on the MCF-7 breast cell lines as compared to ethyl acetate and hexane extract. In addition, the ethanolic extract resulted in the highest radical scavenging activity (61.3% inhibition), while all extracts have shown negligible antiangiogenic activities. Conclusion: The study shows that the ethanolic extract of Mercurialis annua from Jordan could serve as a possible source for developing anticancer and antioxidant drugs in the future.

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Article metadata

Title	Biological Evaluation of Mercurialis annua Extracts for Possible Antioxidant, Antiproliferative and Cytotoxic Activity
Authors	Nedhal Al-Douri; Bilal Ali Al-Jaidi; Hana Mamoun Hammad; Ashok Kumar Shakya; Tahany Jalal Mefleh Alkhalailah; Sireen Abdul Rahim Shilbayeh; Alnada Abdalla Ibrahim; Katharigatta Narayanaswamy Venugopala; Yoonus Thajudeen Kamal
Affiliations	Department of Pharmacy, Al-Manara College of Medical Sciences, Maysan, Al-Amarah, IRAQ.; Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Yarmouk University, Irbid, JORDAN.; Department of Biological Sciences, School of Sciences, University of Jordan, Amman, JORDAN.; Faculty of Pharmacy and Medical Sciences, Al Ahliyya Amman University, Amman, JORDAN.; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Philadelphia University, Amman, JORDAN.; Department of Pharmacy Practice, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh, SAUDI ARABIA.; Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology.; Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, SAUDI ARABIA.; Department of Pharmacognosy, College of Pharmacy, King Khalid University, Abha, SAUDI ARABIA.
Corresponding author	bilal.aljaidi@yu.edu.jo
Journal	Indian Journal of Pharmaceutical Education and Research
Volume / Issue	Vol. 56, Issue 3s (2024)