Comparative HPTLC Analysis of Piperine Content in Roots of Piper longum L. Cultivated with Bio Formulations

Background: Phytochemicals from medicinal plants significantly impact healthcare. Piperine, a bioactive compound in Piper longum, exhibits diverse pharmacological effects, including analgesic, antipyretic, antioxidant, immune-stimulant, hepatoprotective, and bioavailability-enhancing properties. Traditionally, Piperine is extracted from both fruits (Pippali) and roots (Pippalimula) of Piper longum. Objectives: To quantify the Piperine content in Piper longum roots cultivated using bio formulations-Kunapa Jala and Vermiwash-on Piperine yield. Materials and Methods: Piper longum was cultivated with Random Block Design with two treatment groups (Kunapa Jala and Vermiwash), each with 12 replications. Roots were harvested, cleaned, dried, and powdered. High-Performance Thin-Layer Chromatography (HPTLC) was used to detect and quantify of Piperine. Results: HPTLC analysis revealed the presence of Piperine in both groups. Quantitative comparison showed a higher Piperine concentration in the Kunapa Jala treated group than in the Vermiwash group. Conclusion: Kunapa Jala enhances Piperine content in Piper longum roots in comparison to Vermiwash. This supports the potential of Vrukshayurveda and traditional formulations in improving the phytochemical yield of Pippali.