Comprehensive Physico-chemical and HPTLC-Based Standardization of Devdarvyadi Churna: A Classical Ayurvedic Formulation

Background

Devdarvyadi Churna is a classical Ayurvedic polyherbal formulation described by Acharya Charaka for the management of Kaphaja Kasa (cough of Kapha origin). It comprises Devadaru, Haritaki, Musta, Pippali, and Vishva, which possess documented Kapha-Vata-shamak, dipana, pachana, and kasahara properties. Despite its extensive traditional and clinical use in respiratory disorders, systematic scientific validation and standardization data remain limited.

Objectives

The present study aimed to evaluate and standardize Devdarvyadi Churna using contemporary analytical techniques to substantiate its traditional therapeutic claims and establish quality control parameters.

Materials and Methods

Devdarvyadi Churna was prepared according to classical references and standardized procedures. Organoleptic parameters were assessed, followed by physico-chemical analysis including loss on drying, ash values, pH, and extractive values as per the Ayurvedic Pharmacopoeia of India. Preliminary phytochemical screening was conducted to identify major bioactive groups. High-Performance Thin-Layer Chromatography (HPTLC) profiling of the methanolic extract was performed using silica gel 60 F254 plates, with detection at 254 nm and 366 nm to generate characteristic chromatographic fingerprints.

Results

The formulation exhibited acceptable organoleptic and physico-chemical parameters, indicating good quality and stability. Phytochemical screening confirmed the presence of alkaloids, flavonoids, phenolics, tannins, terpenoids, and volatile oils. HPTLC analysis revealed reproducible chromatographic profiles with multiple well-resolved peaks, reflecting the polyherbal complexity of the formulation.

Conclusion

The study establishes reliable standardization parameters and HPTLC fingerprints for Devdarvyadi Churna, scientifically supporting its traditional use in Kaphaja Kasa and enhancing quality assurance and reproducibility.