Open access · Peer reviewed · No APCs
ORIGINAL ARTICLEPharmacognosy ResearchVol. 6 | Issue 4 | 2014 | pp. 312–319Open access
Antiproliferative effects of Plumbago rosea and its purified constituent plumbagin on SK-MEL 28 melanoma cell lines
- 1,
- 2,
- 3,
- 3,
- 3*
- 1 Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar, Tamil Nadu
- 2 Biogenix Research Centre, Trivandrum, Kerala
- 3 Department of Biotechnology, Bharathidasan Institute of Technology Campus, Anna University, Tiruchirappalli, Tamil Nadu, India
Published in Pharmacognosy Research
Correspondence: Sureshkumar Periyasamy
Department of Biotechnology, Bharathidasan Institute of Technology Campus, Anna University, Tiruchirappalli, Tamil Nadu, India
Email: sureshbiotech2003@yahoo.co.in
Copyright: © 2014 Manuscript Technomedia. This is an open access article.
- Published:
- Jan 1, 2014
How to cite
Anuf, A. R., Ramachandran, R., Krishnasamy, R., Gandhi, P. S., & Periyasamy, S. (2014). Antiproliferative effects of Plumbago rosea and its purified constituent plumbagin on SK-MEL 28 melanoma cell lines. Pharmacognosy Research, 6(4), 312–319. https://doi.org/10.4103/0974-8490.138280
Abstract
Background
Plumbago rosea is used in traditional systems of medicine for the preparation of formulations used for treating inflammations, cough, bronchitis, and gastrointestinal disorders, and also in conjunction with cancer chemotherapy. In the present study, the cytotoxic and anti-proliferative effects of plumbagin, and the ethanolic root extract of P. rosea (ETPR) was evaluated on SK-MEL 28 melanoma cell lines and human lymphocytes.
Materials and Methods
MTT and apoptotic assays were used for the evaluation of cytotoxic and anti-proliferative effects, respectively. In addition, the effect of Plumbagin and ETPR in down regulation of BCL-2 expression is investigated using RT-PCR analysis.
Results
Both plumbagin and ETPR dose-dependently decreased the cell viability more potently in melanoma cell lines. P. rosea extract demonstrated significant synergy in inhibiting BCL-2 expression than plumbagin. Moreover plumbagin showed more toxicity in human lymphocytes.
Conclusion
Plumbagin has anti-cancer potential, but the side effects limits its use; yet plumbagin, in combination with other ingredients in Plumbago rosea extract, displays significant synergy leading to a stronger anticancer effect with significantly less toxicity.
Keywords
Subject
Article metadata
| Title | Antiproliferative effects of Plumbago rosea and its purified constituent plumbagin on SK-MEL 28 melanoma cell lines |
|---|---|
| Authors | Alexander Ronaldo Anuf; Rajesh Ramachandran; Rajaram Krishnasamy; P.S. Sudhakar Gandhi; Sureshkumar Periyasamy |
| Affiliations | Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar, Tamil Nadu; Biogenix Research Centre, Trivandrum, Kerala; Department of Biotechnology, Bharathidasan Institute of Technology Campus, Anna University, Tiruchirappalli, Tamil Nadu, India |
| Corresponding author | sureshbiotech2003@yahoo.co.in |
| Journal | Pharmacognosy Research |
| Volume / Issue | Vol. 6, Issue 4 (2014) |
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