Evaluation of Antioxidant and Anti-inflammatory Activities of Selected Cissus Species

Objectives: The present work was aimed at investigating the phytochemicals of methanolic extract of L. procumbens leaves and its isolated mixtures from column fractions. Materials and Methods: The method was successfully developed and two new compounds (chlorogenic acid and 7‑hydroxyflavanone) by liquid chromatography (LC)‑electrospray ionization (ESI)‑MS/MS and four new compounds (lactucin, isorhametin, 1‑monopalmitin, and 1‑hexacosanol) by ultra‑performance liquid chromatography (UPLC)‑ESI‑quad time of flight (QTOF)/MS in L. procumbens were identified. Identification of compounds by LC‑ESI‑MS/ MS and UPLC‑ESI‑QTOF/MS was identified based on the accurate mass of pseudomolecular [M+H]+ ion tandem mass spectrometry (MS/MS) data and by comparing retention times, mass spectra, and molecular weights with those published in the literature. Results: The six new compounds, namely chlorogenic acid, 7‑hydroxyflavanone, lactucin, isorhametin, 1‑monopalmitin, and 1‑hexacosanol were identified by in L. procumbens. Conclusion: A total of 26 compounds by gas chromatography‑MS and five compounds by LC mass‑ESI tandem mass spectrometry (LC‑ESI‑MS/ MS) and seven compounds by UPLC‑ESI tandem mass spectrometry/ quadrupole‑time‑of‑flight‑mass spectrometry (UPLC‑ESI‑QTOF/MS) technique were identified.